whole human genome oligo microarray Search Results


human whole genome oligo microarray service  (Arraystar inc)
90
Arraystar inc human whole genome oligo microarray service
Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to <t>microarray</t> gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.
Human Whole Genome Oligo Microarray Service, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/whole+human+genome+oligo+microarray/pmc07099516-190-40-46?v=Arraystar+inc
Average 90 stars, based on 1 article reviews
human whole genome oligo microarray service - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
whole human genome oligo microarray 4x44k  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation whole human genome oligo microarray 4x44k
Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to <t>microarray</t> gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.
Whole Human Genome Oligo Microarray 4x44k, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/whole+human+genome+oligo+microarray/10__1177_slash_1090820x13482938-44-14-20?v=SuperArray+Bioscience+Corporation
Average 90 stars, based on 1 article reviews
whole human genome oligo microarray 4x44k - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
human oligo microarray phalanx human whole genome onearraytm  (Phalanx Biotech)
90
Phalanx Biotech human oligo microarray phalanx human whole genome onearraytm
Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to <t>microarray</t> gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.
Human Oligo Microarray Phalanx Human Whole Genome Onearraytm, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/whole+human+genome+oligo+microarray/pm22403610-206-39-47?v=Phalanx+Biotech
Average 90 stars, based on 1 article reviews
human oligo microarray phalanx human whole genome onearraytm - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to microarray gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.

Journal: American Journal of Physiology - Cell Physiology

Article Title: MiR-4674 regulates angiogenesis in tissue injury by targeting p38K signaling in endothelial cells

doi: 10.1152/ajpcell.00542.2019

Figure Lengend Snippet: Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to microarray gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.

Article Snippet: For DNA microarray gene chip analysis, HUVECs were transfected with 30 nM miRNA negative control or miR-4674 mimics for 24 h. Cells were collected into RNeasy mini kit (Qiagen) and sent for two-color, 4 × 44 K format (Agilent Technologies) human whole genome oligo microarray service (ArrayStar).

Techniques: Transfection, Negative Control, Microarray, Real-time Polymerase Chain Reaction, Western Blot, Immunoprecipitation, Quantitative RT-PCR, Expressing, Luciferase, Activity Assay